Simultaneous and Accurate Determination of Water- and Fat-soluble Vitamins in Multivitamin Tablets by Using an Rp-hplc Method
نویسندگان
چکیده
In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R > 0.999) over the concentration range of 10–500 ng mL. The statistical evaluation of the method was carried out by performing the intraand inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.
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